Teaching Schedule

Teaching Schedule / Attività didattica Dottorato SVA 2017-2018

Date Day Course Time Room Lecturer
21/11/2017 Tuesday competenze trasversali

09.00-13.00

G24  
14/11/2017 Tuesday Medical Statistic 2

09.30-13.30

310 Paola Crepaldi
28/11/2017 Tuesday Medical Statistic 2 09.30-13.30 311 Paola Crepaldi
05/12/2017 Tuesday Medical Statistic 2 09.30-13.30   Paola Crepaldi
12/12/2017 Tuesday Medical Statistic 2

09.30-13.30

311 Paola Crepaldi
19/12/2017 Tuesday Medical Statistic 2

09.30-11.30

  Paola Crepaldi
24/01/2018 Wednesday

Communication 2

09.00-13.00   Ettore Galanti
07/02/2018 Wednesday

Communication 2

09.00-13.00   Ettore Galanti
21/02/2018 Wednesday Communication 2 09.00-13.00   Ettore Galanti
05/04/2018 Thursday Experimental Pathology
09.00-12.00 Biblioteca anatomia patologica Eugenio Scanziani
12/04/2018 Thursday Experimental Pathology 09.00-12.00 Biblioteca anatomia patologica Eugenio Scanziani
19/04/2018 Thursday Experimental Pathology 09.00-12.00 Biblioteca anatomia patologica Eugenio Scanziani
26/04/2018 Thursday Experimental Pathology 09.00-12.00 Biblioteca anatomia patologica Eugenio Scanziani

Minoli/Scanziani

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PhD Student: Lucia Minoli

Lucia Minoli graduated with honours  in 2016 in Veterinary Medicine at the University of Milan with a thesis on preclinical animal models for human ovarian cancer therapy. Since 2013 she has pursued an internship at the Mouse & Animal Pathology Lab (Filarete Foundation, Milan) as an intern student, where she has been trained as an anatomic and experimental pathologist. In 2015 she was enrolled at the same laboratory as a trainee in histological diagnostic of small animals tumors. After her graduation she spent a six month-period at Edmund Mach Foundation (TN), working on epidemiological distribution of Mycobacterium microti in wild rodents.

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Tutor: Prof. Eugenio Scanziani

Eugenio Scanziani graduated with honours in Veterinary Medicine in 1979 from the University of Milan. From 1984 he is researcher/associate professor/full professor of Veterinary Pathology at the Faculty of Veterinary Medicine of Milan. From 1995 he is Charter Member of the European College of Veterinary Pathologists (ECVP). During 1997-2002 he is member of the Examination Board of the ECVP. From 2004 to 2005 he is the President of the Italian Association of Veterinary Pathology (AIPVet). From 2007 to 2010 he is the Dean of the Faculty of Veterinary Medicine of Milan. From 2009 to 2013 he is member of the “Consiglio Superiore di Sanità”, Ministry of Health, Rome. From 2008 he is the Head of the Mouse & Animal Pathology Lab (MAPLab) of Filarete Foundation.
His research area in the field of veterinary pathology is focused on the study of animal models for human diseases and pathogenesis of bacterial diseases in domestic animals. Eugenio Scanziani has published 158 peer reviewed articles (indexed in PubMed) in internationally recognized journals. His h index is 30 (ISI Web of Science).

 

PROJECT  DESCRIPTION: 

Tumor microenvironment in experimental models of human cancer

Over the past decade, our understanding of tumors have drastically changed: tumors are no longer considered as a growth of homogeneous neoplastic cells, but as an actual organ composed of different structures. Concurring to this complex entity are, apart from neoplastic cells, numerous cell populations and structures (like tumor-associated vasculature, immune/inflammatory cells, fibroblast…) that could be all-together referred to as tumor microenvironment. Tumor microenvironment has a dual role in tumor biology, both promoting and antagonizing tumor growth. Accordingly, it has a great potential as a target for novel therapeutic approaches.

Experimental animal models represent a widely used tool in the study of human diseases, thanks to recapitulating pathogenetic and pathological features of different diseases. In particular, orthotopic xenotransplantation of human tumors into immunocompromised mice allows to reproduce the tumor microenvironment and mimics biological behavior of the corresponding human tumor.

Our project, that will be carried out in collaboration with different Institutions, will utilize different tumoral models with the goals of investigating tumor microenvironment and identifying biological processes that regulate tumor development and progression. These data will provide a support to future advances in therapies. Our project will be carried out relying on a multidisciplinary approach, including clinical, biochemical and molecular analyses. In particular, our main contribution will be focused on histological and immunohistochemical assays, which enable to perform quali-quantitative analyses to investigate the different components and morphological changes that occur in tumor.

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Fig. 1 – Mouse, pancreas. Orthotopic patient derived xenograft of pancreatic ductal adenocarcinoma, immunohistochemical staining with anti-human class I major histocompatibility complex (human MHC I), 100x. Ihc staining show the human origin of neoplastic cells (positive for anti-human MHC I) surrounded by a host-derived stromal support (negative for anti-human MHC I).

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Fig. 2 – Mouse, subcutis, patient derived xenograft of human ovarian carcinoma. Immunohistochemical staining with anti-CD31, immunopositive vascular outlines (100x).

 

 

 

Perricone/Agazzi

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PhD Student: Vera Perricone

She graduated with honors in July 2016 in Veterinary Medicine at University of Milan, discussing a thesis entitled “Dietary supplementation of beta-alanine in weaned piglets: effects on growth performance, serum biochemistry and plasma concentration of amino acids” (Supervisor: Prof. Valentino Bontempo). During her career she spent one year abroad, in Spain, at the Veterinary Faculty of Cordoba, attending at the veterinary hospital. Her research is primarily concerned with animal nutrition, the study of new feeds and nutritional technologies for higher health and sustainability of livestock products.

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Tutor: Dr. Alessandro Agazzi

Dr. Alessandro Agazzi is researcher in Animal Nutrition from 2006 at the Università degli Studi di Milano- Department of Health, Animal Science and Food Safety (VESPA). He has published 38 papers on international scientific journals with impact factor and these publications have been cited more than 190 times with an H-index of 8. The general topic of his work is the role of nutrition of both monogastric and poligastric animals on performance and health status. In recent years the research activity has focused on the efficacy of natural compounds, growth promoter alternatives, and fatty acids sources as mediators on inflammation, immune response and gut health with special regard to polyunsaturated fatty acids.

For more information, see: 

http://www.unimi.it/chiedove/cv/alessandro_agazzi.pdf
http://www.vespa.unimi.it/ecm/home

 

PROJECT DESCRIPTION:

Agricultural resources will be not enough to provide food for everyone in the next future and are still affected from poor sustainable technologies. Feeding the world with renewable, low-impact, high-value feed and food sources and lower the impact of animal productions by new technologies is the challenge for the future. Conventional feed sources in livestock nutrition leads to competition between animals and human, to solve this problem improved sustainability and productivity of terrestrial livestock systems are due. At the moment the nutritional regimes for milk and meat production are based on not environmental friendly vegetable or animal-derived sources; in such conditions alternative, renewable and environmental-friendly feed sources are needed to lower costs for animals rearing and milk and meat production. In the same way new feeds, such as specific nutritional additives, can support increased nutrient efficiency and animal health status, decreasing both the production costs of livestock rearing and medications. On the other side the improvement or the development of new nutritional technologies could match the target of a higher sustainability of production cycles, assuring optimal nutrient availability at a higher efficiency rate and lower costs. Both new or alternative feeds and available innovative nutritional technologies are strictly connected each others, being often the new technologies applied to perform a new feed or nutritional compounds or being used to improve the nutritional effect of the feeds. In this view at the moment the more efficient, sustainable and economical approaches still are not identified and a large research area is available. The aim of the project is to evaluate use of alternative feeds to conventional sources, innovative feeds and technologies for higher sustainability in livestock, through improved performance and health status.

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Trovatelli/Acocella

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Phd Student: Marco Trovatelli

Marco Trovatelli graduated in Veterinary Medicine at the University of Milan in February 2016, with thesis entitled “Evaluation of the depth of penetration into abdominal wall of Abraxane after hyperthermic intraperitoneal administration (HIPEC) in rabbit”. He obtained the medical habilitation to professional practice in June 2016. During his university course he performed an internship at Veterinary Teaching Hospital of Università degli Studi di Milano, Surgical division. He participated, like official visitor, in a multi organ transplant procedure from a brain death patient at Spedali Civili di Brescia of Università degli Studi di Brescia, 3^ General Surgical division. His main interest fields are in surgery and experimental medicine. Now he is working at the Unit of Veterinary Surgery, Department of Health, Animal Science and Food Safety where he started a PhD program in October 2016 with a research project in “Animal model for minimally invasive neurosurgery”.

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Tutor: Prof. Fabio Acocella

After his degree in Veterinary Medicine at the University of Milan in 1991, Fabio Acocella obtained a PhD at University of Milan in 1998. After a period as contract professor in Anatomy of the Domestic Animals at University of Padova, he received a postdoc in fetal surgery at University of Milan from 1998 to 2002. He has been part of a teamwork to establish the Experimental Surgery Center for didactic and research at Univesité des Montagnes, Bangangté (Cameroon) in 2003-2006. He became researcher at University of Milan in 2006 and then aggregate professor in surgery until 2012. Now he is currently associate professor of Veterinary Surgery at Department of Health, Animal Science and Food Safety at University of Milan. In 2008 he has been a visiting scientist at Institute of Regenerative Medicine (Wake Forrest University, NC, USA) and in 2014 at Transplant Immunology Laboratory (Stanford, Ca, USA). His main interests are in general and thoracic surgery, regenerative medicine, experimental models in surgery and education in surgery. He is author of 26 international publication peer-review and of two book chapters in regenerative medicine area.

PROJECT DESCRIPTION:

Minimally invasive surgery has been considered a major revolutionary method in surgery. Although all the advantages and the benefits of minimally invasive surgeries, not all surgeries can be done invasively. Robotically-assisted surgery overcomes the restricted mobility problems by performing the normal surgery movements although the robotics arms in fact carry out the movement using end-effectors and manipulators to do the actual surgery on the patient. Neurosurgery has witnessed an accelerated growth in the advancement and clinical adoption of Computer Tomography (CT) and Magnetic Resonance Imaging (MRI). Diffusion tensor imaging (DTI) is a form of diffusion weighted MRI that assesses physiological water directionality and motion, providing images of important white matter tracts within the Central Nervous System (CNS). The rapid evolution in clinical approach has influenced many areas of neurosurgery but there are still diseases without a standard of care. Gliobastoma (GBM) is one of these diseases which can’t be complete neurosurgical removed. As a result, surgery must be followed by adjuvant therapy modalities like radiotherapy (RT), chemotherapy (ChT) like convection-enhanced delivery procedure (CED), or both. The procedure still suffers from significant shortcomings, like reflux, inaccurate catheter placement and limited drug distribution within the substrate at the point of delivery. The project is focused on the use of a minimally invasive approach with robotic control. Sheep, like animal model, will be used to improve the use of CED, passing the problems like damage of a rigid catheter or the inaccurate catheter placement and limited drug distribution.

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Figure 1: Diffusion tensor based images

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Figure 2: Glioblastoma multiforme

Gioeni/Ravasio

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PhD Student: Daniela Gioeni

Daniela Gioeni graduated in October 2015 in Veterinary Medicine at the university of Milan with full mark and honour, with a final dissertation concerning dog anaesthesia entitled “Transmucosal administration of dexmedetomidine in dogs: effect of preanesthetic sedation in combination with butorphanol or methadone" (Supervisor: Prof. Diego Fonda; assistant supervisor Dr. Giuliano Ravasio). She attended the Anaesthesia unit at the Department of Veterinary Sciences and Public Heath (University of Milan, Italy) for three years before graduation as an intern student. She also spent one year in the Neuroradiology Unit, preclinical research department of IRCSS “Carlo Besta Hospital”. From Jenuary 2016 she has started working as an anaesthetist at the “San Francesco Veterinary Hospital” in Milan. During the last year she participated to Association of Veterinary Anaesthetist (AVA) meetings in Prague and Lyon as a co-author of 4 research projects. Her main interests are veterinary anaesthesia and critical care and she has a strong passion for the research field. Her research interest is mainly focused on the relationship between anaesthesia protocol and brain connectivity and alteration in brain perfusion.

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Tutor: Prof. Giuliano Ravasio

The main purpose of my study and research is to dedicate myself to veterinary anaesthesia and analgesia. Specifically, I am interested in clinical efficacy, safety, pharmacokinetics and pharmacodynamics of systemically administered analgesic drugs such as NSAIDs in cats and dogs and opioid in horses, cats and in dogs. I developed new regional anaesthesia technique in dogs through neurolocation by use nerve locator. I tested the clinical efficacy and safety of different local anaesthetics (ropivacaine, lidocaine and bupivacaine) and an opioid through different routes of administration such as epidural and perineural in dogs or administered through the multiperforated catheter implantation in the surgical wound for the continuous infusion of local anaesthetics in post-operative pain relief after surgical cancer excision in cats. I verified the effectiveness of electroacupuncture (particularly important in human medicine and actually very interesting in veterinary medicine) in a canine and equine model.  With respect to general anaesthetics, I verified clinical efficacy and safety of some general anaesthetics drugs such as ketamine, propofol and dexmedetomidine in animal. Today I work with several research institutes, such as Neurological Institute “Carlo Besta” and San Raffaele Hospital (“Università Vita-Salute”) on laboratory animal research, with my personal contribution as veterinary anaesthesia specialist. 

 

PROJECT DESCRIPTION:

Advanced magnetic resonance imaging (MRI) techniques have been used with increasing frequency to investigate the central nervous system (CNS). In order to obtain a good quality imaging, immobility must be achieved both in human and in animal patients. For this reason, anaesthesiologist assistance is essential and the analysis of the drugs impact on neuronal and hemodynamic response becomes critical. In particular, it is crucial to understand the modification induced by different sedative drugs on global and regional cerebral blood flow (CBF) and on contrast media biodistribution in order to obtain a better management of patients with neurological disease that require MRI examination. Currently, only few studies have investigated the effects of different kind of anaesthetic on cerebral perfusion and brain functional connectivity. Furthermore, the study of functional brain connectivity is a focal point in preclinical research in order to investigate a wide variety of neuroscience research questions including neurovascular relationship, pain mechanism, consciousness and longitudinal functional development and re-organization. The purpose of this study is to evaluate the influence of different sedative/anaesthetic protocols (isofluorane, dexmedetomidine, midazolam and thiopental sodium) on brain perfusion, using gadolinium DSC-MRI (Prohance vs Multihance) and brain functional connectivity rising from the BOLD signal studied with fMRI. The study will be performed with a high field 7 Tesla magnetic resonance system. The secondary aim is to obtain data that can improve anaesthetic management both in animal practice and human clinical setting in order to extend the knowledge about sedative drugs mechanism within brain.

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