Gambini/Caniatti

Matteo Gambini graduated with honours in Veterinary Medicine in October 2016 at the University of Perugia with a thesis on Borna disease and Borna-like encephalitis in Italy. Before and after his graduation, he spent several months as a voluntary intern in veterinary anatomical pathology at his home University, being involved in diagnostic activities. From January to September 2017, he took part in an Erasmus+ Traineeship at the Department of Pathology of University of Veterinary Medicine Hannover, performing research on viral oncolysis in a murine translational model, and training in lab animal management and several lab techniques.

Degree in Veterinary Medicine in 1985 and PhD in “Comparative Patholoy of Domestica Animals” in 1990 (Università degli Studi di Milano).From 1998 Diplomate of the European College of Veterinary Pathology. From 2000 in charge of the “Veterinary Diagnostic Cytology Service” at Veterinary Faculty of Milano. From 2001 Associate Professor in Veterinary Anatomic Pathology. From 2016 he is unit head of the Anatomic Pathology Unit of the Milano University Veterinary Hospital. Research activity focused on diagnostic cytology, cutaneous neoplasia, lymphoproliferative neoplasia and chronic nasal disease. Caniatti is co-author of scientific publications in peer-reviewed international journals (ORCID ID: 0000 0003 3541 1339) and is a congresses and courses invited speaker.

 

Title: Lymphoid and histiocytic cells in inflammatory and neoplastic microenvironment

The main function of immune system is to recognize non-self antigen and to protect the host against pathogens and development of cancer. Protection activities are carried out through effector mechanisms. These can be either beneficial or harmful. Indeed, almost every immune response against pathogenic agents gives rise to pathologic events, primarily inflammation. The latter can be successful in the elimination of foreign pathogens, or fails resulting in chronic inflammation or even misdirected immune response (i.e. hypersensitivity reactions and autoimmune disease). Immune response plays a dual role also in tumor microenvironment, promoting or antagonizing tumor development. Indeed, chronic inflammatory conditions could represent one of the main triggers of tumorigenesis. Moreover, the immune response is capable of improving tumor immune evasion through immunoediting. On the other hand, an immune response directed against tumor-specific and associated antigens could be involved in neoplasm regression (i.e. immunosurveillance). Therefore, a deep understanding of the correlations that exist between immune response and neoplastic cells is fundamental for setting new therapeutic approaches (i.e. immunotherapy).

Lymphocytes and histiocytes represent the main cells involved in chronic immune responses against non-self antigens associated with pathogenic persistent microorganisms and neoplasms. The aim of this project is the investigation of roles and features of these particular immune cells associated with chronic inflammatory conditions and neoplasms in veterinary medicine with a special emphasis on pathologic events that affect lymph nodes and spleen. This study will be carried out on both histologic and cytologic specimens.  According to the fixation method used for each tissue (fresh, frozen and FFPE), samples will be submitted to routine histologic and/or cytologic examination, immunohisto-/immunocytochemical analysis and microarray assays to characterize lymphocytes (e.g. B lymphocytes vs T lymphocytes) and histiocytes (e.g. M1-polarized VS M2-polarized) associated with chronic inflammatory events and neoplasms. Obtained data will be correlated with prognosis and clinical outcome, providing support to future advances in therapeutic approaches.

Fig.1 – Dog, skin scraping. Large  multinucleated macrophage engulfing fungal structures. Lymphoid cells are present (100x; MGG).

 

Fig.2 – Cat, abdominal effusion. Pleomorphic population of large immature granulated lymphoid cells. Large Granular Lymphoma (LGL) (100x; MGG).