Gioeni/Ravasio

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PhD Student: Daniela Gioeni

Daniela Gioeni graduated in October 2015 in Veterinary Medicine at the university of Milan with full mark and honour, with a final dissertation concerning dog anaesthesia entitled “Transmucosal administration of dexmedetomidine in dogs: effect of preanesthetic sedation in combination with butorphanol or methadone" (Supervisor: Prof. Diego Fonda; assistant supervisor Dr. Giuliano Ravasio). She attended the Anaesthesia unit at the Department of Veterinary Sciences and Public Heath (University of Milan, Italy) for three years before graduation as an intern student. She also spent one year in the Neuroradiology Unit, preclinical research department of IRCSS “Carlo Besta Hospital”. From Jenuary 2016 she has started working as an anaesthetist at the “San Francesco Veterinary Hospital” in Milan. During the last year she participated to Association of Veterinary Anaesthetist (AVA) meetings in Prague and Lyon as a co-author of 4 research projects. Her main interests are veterinary anaesthesia and critical care and she has a strong passion for the research field. Her research interest is mainly focused on the relationship between anaesthesia protocol and brain connectivity and alteration in brain perfusion.

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Tutor: Prof. Giuliano Ravasio

The main purpose of my study and research is to dedicate myself to veterinary anaesthesia and analgesia. Specifically, I am interested in clinical efficacy, safety, pharmacokinetics and pharmacodynamics of systemically administered analgesic drugs such as NSAIDs in cats and dogs and opioid in horses, cats and in dogs. I developed new regional anaesthesia technique in dogs through neurolocation by use nerve locator. I tested the clinical efficacy and safety of different local anaesthetics (ropivacaine, lidocaine and bupivacaine) and an opioid through different routes of administration such as epidural and perineural in dogs or administered through the multiperforated catheter implantation in the surgical wound for the continuous infusion of local anaesthetics in post-operative pain relief after surgical cancer excision in cats. I verified the effectiveness of electroacupuncture (particularly important in human medicine and actually very interesting in veterinary medicine) in a canine and equine model.  With respect to general anaesthetics, I verified clinical efficacy and safety of some general anaesthetics drugs such as ketamine, propofol and dexmedetomidine in animal. Today I work with several research institutes, such as Neurological Institute “Carlo Besta” and San Raffaele Hospital (“Università Vita-Salute”) on laboratory animal research, with my personal contribution as veterinary anaesthesia specialist. 

 

PROJECT DESCRIPTION:

Advanced magnetic resonance imaging (MRI) techniques have been used with increasing frequency to investigate the central nervous system (CNS). In order to obtain a good quality imaging, immobility must be achieved both in human and in animal patients. For this reason, anaesthesiologist assistance is essential and the analysis of the drugs impact on neuronal and hemodynamic response becomes critical. In particular, it is crucial to understand the modification induced by different sedative drugs on global and regional cerebral blood flow (CBF) and on contrast media biodistribution in order to obtain a better management of patients with neurological disease that require MRI examination. Currently, only few studies have investigated the effects of different kind of anaesthetic on cerebral perfusion and brain functional connectivity. Furthermore, the study of functional brain connectivity is a focal point in preclinical research in order to investigate a wide variety of neuroscience research questions including neurovascular relationship, pain mechanism, consciousness and longitudinal functional development and re-organization. The purpose of this study is to evaluate the influence of different sedative/anaesthetic protocols (isofluorane, dexmedetomidine, midazolam and thiopental sodium) on brain perfusion, using gadolinium DSC-MRI (Prohance vs Multihance) and brain functional connectivity rising from the BOLD signal studied with fMRI. The study will be performed with a high field 7 Tesla magnetic resonance system. The secondary aim is to obtain data that can improve anaesthetic management both in animal practice and human clinical setting in order to extend the knowledge about sedative drugs mechanism within brain.

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