Nordio / Giudice

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Laura Nordio graduated in Veterinary Medicine in July 2014 at the University of Padova with final marks of 110/110 with honors. After a period as intern at the section of veterinary anatomical pathology, she discussed a thesis entitled “Immunoistochemical and histopathological study of pancreatic lesions in turkeys experimentally infected with avian influenza viruses”. The results of the study were then published as a paper on Avian Pathology.
As a postgraduate, she spent a 6-months training period at the Istituto Zooprofilattico Sperimentale delle Venezie dealing mainly with diagnostic activities. In October 2015 she enrolled as PhD student at the University of Milan with a project on the histological and molecular diagnosis of canine and feline melanocytic tumors.

Chiara Giudice is a Researcher at the School of Veterinary Medicine of the University of Milan. She obtained her PhD from the University of Milan, 2001, with a thesis on ocular pathology, after a period spent in the COPLOW laboratory (held by Prof. RR Dubielzig) of the Veterinary Medical School of Wisconsin. She ECVP diplomated in 2009. Her research interest is mostly focused on veterinary ocular pathology and comparative oncology. She is currently staff member of the diagnostic laboratory of the Department where she is on duty for routine histopathology (comparative oncology) and runs her personal service for ocular pathology diagnostic.
Dr. Giudice is an active member in professional societies like ESVP and ECVP; SOVI (Italian Society of Veterinary Ophthalmologist); AIPVET (Italian Association of Veterinary Pathologists). She is a member of the Faculty PhD Board and member of the Departmental (DIVET) Commission for Research.
Research interests
Comparative Ocular pathology
Comparative Oncology
Pathology of the Ear in the domestic species

Research project
Prognostication markers in canine and feline melanomas: a comparative study

Melanomas are among the most important malignancies both in human beings and in animals.
Despite numerous efforts of the scientific community in the last decades, melanoma is still a challenge both from a diagnostic and from a prognostic point of view.
Increased availability of specific markers in the late years allowed more precise diagnosis of amelanotic melanomas but prognostication criteria, especially in veterinary medicine, are still unclear. Histological grading systems, based on the evaluation of morphological criteria (e.g anysokariosis or mitotic rate), are mostly insufficient to define a prognosis. This is especially true in the study of uveal melanomas, one of the most aggressive cancer in human beings.
Most recent studies in human and veterinary pathology have focused on the investigation of somatic genetic mutation in melanoma cell lines and neoplastic tissue and have correlated the expression of some genes (e.g 5HT2RB) with malignant phenotype but also with documented metastatic potential.
The aims of the present study are therefore
1) to investigate the expression of genes related to metastatic potential in canine and feline melanomas by Reverse Transcription- PCR
2) to investigate the possibility of retrieving information on gene expression from formalin fixed paraffin embedded tissue. The feasibility of the use of FFPE tissue in the study of gene expression would allow to access archive material and to perform retrospective studies on a larger cohort of cases and on rarer forms of the disease.
3) to investigate immunohistochemical localization of involved gene products in neoplastic tissues.

Although molecular techniques are increasingly diffuse and accessible, immunohistochemistry is still one of the cheapest and easiest methods for the investigation of neoplasia. Moreover immunohistochemistry allows tissue localization of the investigated antigens, with possible insight in specific cell functions.