Bolis / Veronesi

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Barbara Bolis graduated in Veterinary Medicine in October 2013 with a dissertation tesi entitled: ”IGF-1, NEFA and cortisol concentrations in fetal fluids of term pregnancy dogs”, whose data were collected during the three years internship at the Division of Veterinary Obstetrics and Gynaecology, Veterinary Medicine, Milan, Italy and with the cooperation with Dr Alessandro Rota, ECAR Resident, practitioner in Bergamo, Italy. She stayed at the Faculty of Veterinary Medicine of Teramo during 1-28 February 2014 and at the Laboratory of the Section of Veterinary Physiology and Animal Nutrition, University of Udine during 1 April-30 June 2014. Barbara Bolis main interest is for small animals reproduction and especially for small animals neonatology.


Maria Cristina Veronesi, DVM, PhD, ECAR dipl, Ass Prof, Milan, Italy
Graduated in Veterinary Medicine in 1993 (110/110 cum laude), PhD in 1997, Researcher from 1998, ECAR Diplomate in 2002, Associate Professor from 2005 at the Division of Veterinary Obstetrics and Gynaecology, Veterinary Medicine, Milan, Italy. Teacher of several academic and post graduate courses about domestic animals reproduction and neonatology at the University of Milan, of post graduate courses at the University of Teramo and of several private cultural associations for veterinary practitioners. From 2010 she is responsible for the section of Reproduction for the “Vetpedia”, the on-line veterinary medicine encyclopedia, and she is also member of the Italian Group of Small Animals Pediactrics Study (GISPEV) Board. She is also reviewer for European PhD projects evaluation, PhD thesis evaluation, and for International Scientific Journals.
Author of more than 160 publications on International peer-reviewed journals, International and National congresses, in 2005 she was co-author of the book entitled: “Veterinary Neonatology”, as author of the section of “Dogs and cats neonatology”.
Active as clinician at the Division of Veterinary Obstetrics and Gynaecology, Veterinary Medicine, Milan, Italy from 1998, her research interest cover all the fields of domestic animals reproduction, mainly focused on domestic animals neonatology. Recently the research interest was particularly addressed to the use of alternative matrices for the study of small animals perinatology by non-invasive research methods.


Despite the full interest in horses and large animals, veterinary medicine and researches addressed to the study of small animals perinatal development and well-being is still limited, mainly because of difficulties related to the invasiveness of the most techniques of study. However, the knowledge of the last period of antenatal development and the neonatal adaptation should be of great impact for scientific purpose, and especially for the best clinical management of periparturient bitches and queens and newborn puppies and kittens. It is indeed reported that in dogs and cats perinatal losses could be greater than 30% and deserve therefore a full investigation for a successfully reproductive process even in these species.


The investigation on fetal and neonatal physiology implies often the need of invasive serial blood samples collection by both fetuses/newborn and by the mother, not acceptable from an ethical or an animal welfare perspective.



Figure 1:  Fetal extraction during Caesarean section in a dog

For these reasons, in agreement with the need of new, alternatives, non-invasive methods for dogs and cats perinatal study, the project is aimed to assess the full composition of fetal fluids collected during routine elective caesarean section (figure 1) in term pregnant dogs and cats, as well as, to check the possibility of using new non invasive matrices, such as neonatal hair and nails, for long time-frame, retrospective assessment of hormonal accumulation during the last antenatal and the neonatal period.


The study will be aimed to describe the picture in normal, viable and mature puppies (figure 2) and kittens. In addition the study will be also aimed to assess possible differences between normal, healthy newborns as compared to less viable, diseased newborns, providing useful diagnostic and/or prognostic tools for the evaluation of fetal/neonatal well-being and diseases or development abnormalities, leading to a better management of newborns also in small animals medicine.

Figure 2: Clinical assessment of newborn maturity, absence of gross malformations and vitality during Caesarean section in a dog